determine the sequence below t
B. The name of the gene that will be sequenced to find outunknown strain of E.coli will be 16S Ribosomal RNA gene.Apartial sequence of which will be amplified to send forsequencing.
C. Instead of targeting the entire genome of the bacteria this16S rRNA gene is targeted because they are ubiquitous in naturewhere ribosomes can not translate mRNA if there is no 16S rRNAcomponent. Hence they are very essential in prokaryotes and alsohigly conserved. So we can construct universal primers for thesehighly conserved region and can amplify 16S rRNA gene fromdivergent bacteria.
D. The size of 16S rRNA gene is around 1400 bp and present inmultiple copies hence very easy to amplify to get prominentband.
Conserved regions : These are the similar sequence that remainedunchanged which would help us to make phylogenetic relationshipsamong different species.
The technique used for the amplification of this regionis PCR. The primers do bind to conserved region not tovariable region. However the sequence amplified which also containvariable region help to distinguish between 2 species.