METHODOLOGY WITH IMAGE WHAT IS
METHODOLOGY WITH IMAGE
WHAT IS THE PROCEDURE OF STEAK PLATE INOCULATION TECHNIQUES
HOW TO CALCULATE COLONY
HOW TO APPLY GRAMS STAINING
DISCUSS ABOUT BACTERIA DIFFERENTIATION TECHNIQUES
IMPORTANT POINT OF LITERATURE METHODOLOGY AND DISCUSSIONPARTS
Answer:
Procedures of Streak Plate InnoculationTechniques are:
1. Sterilize the innoculating loop untill it isred hot and cool it.
2. Insert the loop into tube/ culture bottle and remove someinnoculum.
3. Immediately streak the innoculating loopvery gently over a quarter of plate using back and forthmotion.
4. Flame the loop and cool again,extend the streaks into thesecond quarter of the plate.
5. Repeat step 4 to streak into the 3rd quarter and centerfourth area of the plate.
Colony are calculatedon the basisof:
1. All colonies should have the samegeneral appearance.
2. If there is more than one type, each colony should bestreaked again.
Procedures of gramstaining:
1. Apply a primary stain ( crystal violet) to hot- fixedbacterial culture.
2. Addition of Iodide, binds to Cristal violet and holds it inthe cell.
3. Rapid decolourisation by acetone or ethanol.
4. Counterstaining with safranine.
Bacteria differentiation techniques: Bio-, sero-and phagetyping,antibiotic susceptibility test, immunoblotting, polyacrylamide gelelectrophoresis,etc.
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